Defining Essential Genes for Human Pluripotent Stem Cells by CRISPR-Cas9 Screening in Haploid Cells

Yilmaz, Peretz et al., April 16, 2018

Summary: The maintenance of pluripotency requires coordinated expression of a set of essential genes. Using our recently established HhPSCs, we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs.